Hugonia mystax L. is an evergreen woody liana distributed throughout India, in dry forest area. Traditionally, the leaves were used for anthelmintic and rheumatism. The present investigation was analyzed to evaluate the pharmacognostical studies on selected medicinal plant for the first time in this plant. The parameters such as anatomical, histochemical colour reactions, fluorescence analysis, ash values, extractive values were done in the leaves. The values calculated and data collected could be used for the identification of powdered drug of this taxon. Pharmacognostical analysis will help to identify authentically the drug from adulteration, substitution in the herbal market and also for the quality control in pharmaceutical industry.